Journal: Journal of Neuroinflammation
Article Title: Macrophages in trigeminal ganglion contribute to ectopic mechanical hypersensitivity following inferior alveolar nerve injury in rats
doi: 10.1186/s12974-017-1022-3
Figure Lengend Snippet: Effect of intra-TG etanercept administration on mechanical allodynia following IANX. a The timing of the cannula implantation, etanercept, or vehicle administration into the TG, IANX, or sham operation (upper) and changes in MHWT of the ipsilateral whisker pad skin by daily etanercept administration into TG following IANX (lower). The MHWT was normalized to the pre-IANX MHWT (100%). Arrows indicate the timing of intra-TG etanercept or vehicle administration. Error bars indicate SEM. ** p < 0.01 vs. IANX + vehicle ( n = 6 in each; two-way ANOVA with repeated measures followed by Bonferroni’s multiple-comparison test). b TNFR1 immunoreactivity in ipsilateral TG on day 3 following IANX or in sham-operated rats. Arrows indicate FG-labeled TNFR1-IR neurons. c Changes in the mean percentage of FG-labeled TNFR1-IR neurons on day 3 following IANX. Error bars indicate SEM. * p < 0.05 vs. sham ( n = 5 in each, Student’s t test)
Article Snippet: After rinsing with 0.01 M PBS three times for 5 min each, sections were incubated in mouse anti-Iba1 polyclonal antibody (1:200; Abcam, Cambridge, MA, USA) and rabbit anti-TNFα monoclonal antibody (1:200; Abcam) or rabbit anti-glial fibrillary acidic protein (GFAP) polyclonal antibody (1:500; cat.No. ab7260, Abcam) or rabbit anti-F4/80 monoclonal antibody (1:500; cat.No. ab111101, Abcam), or anti-tumor necrosis factor receptor-1 (TNFR1) monoclonal antibody (1:50; Santa Cruz, Santa Cruz, CA, USA), diluted in 0.01 M PBS containing 4% normal donkey serum (Merck, Darmstadt, Germany) in 0.3% Triton X-100 (Merck) overnight at 4 °C.
Techniques: Whisker Assay, Comparison, Labeling